Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.
KLH-conjugated linear peptide corresponding to Threonine.
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.
Research Sub Category
General Post-translation Modification
This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.
Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Calyculin/okadaic acid treated and untreated A431 cell lysates.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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