05-257

Sigma-Aldrich

Anti-p300 CT Antibody, clone RW128

clone RW128, Upstate®, from mouse

Synonym(s):
KAT3B
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RW128, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

application(s)

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... EP300(2033)

General description

p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
p300 and CBP are thought to increase gene expression in three ways:
(1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
(2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
(3) by acting as adaptor molecules.

Specificity

Recognizes p300 and co-immunoprecipitates E1A.

Immunogen

Epitope: C-terminus
GST-fusion to the C-Terminus of human p300

Application

Research Category
Epigenetics & Nuclear Function
Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.
Research Sub Category
Histones

Chromatin Biology

RNA Binding Protein (RBP)

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Target description

300 kDa

Physical form

Protein G Purified
Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Control Included: HeLa Nuclear Extract (12-309)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Marco Turci et al.
Retrovirology, 9, 102-102 (2012-12-12)
Retroviruses HTLV-1 and HTLV-2 have homologous genomic structures but differ significantly in pathogenicity. HTLV-1 is associated with Adult T cell Leukemia (ATL), whereas infection by HTLV-2 has no association with neoplasia. Transformation of T lymphocytes by HTLV-1 is linked to...
Surabhi Dangi-Garimella et al.
PloS one, 8(5), e64566-e64566 (2013-05-23)
Pancreatic ductal adenocarcinoma (PDAC) is associated with a pronounced collagen-rich stromal reaction that has been shown to contribute to chemo-resistance. We have previously shown that PDAC cells are resistant to gemcitabine chemotherapy in the collagen microenvironment because of increased expression...
Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy.
Franklin, S; Zhang, MJ; Chen, H; Paulsson, AK; Mitchell-Jordan, SA; Li, Y; Ping, P; Vondriska, TM
Molecular and Cellular Proteomics null
MAPK p38 regulates transcriptional activity of NF-kappaB in primary human astrocytes via acetylation of p65.
Saha, RN; Jana, M; Pahan, K
Journal of immunology (Baltimore, Md. : 1950) (1950)
Targeting of aryl hydrocarbon receptor-mediated activation of cyclooxygenase-2 expression by the indole-3-carbinol metabolite 3,3'-diindolylmethane in breast cancer cells.
Degner, SC; Papoutsis, AJ; Selmin, O; Romagnolo, DF
The Journal of Nutrition null

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