05-386

Anti-Vinculin Antibody

UPSTATE^®, mouse monoclonal, V284

• Synonym(s): Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: V284, monoclonal
• Species reactivity: human, rat, mouse, rabbit, chicken
• Application: IHC, IP, WB
• Citations: 95

Properties

• Product Name: Anti-Vinculin Antibody, clone V284, clone V284, Upstate^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: V284, monoclonal
• species reactivity: human, rat, mouse, rabbit, chicken
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_003373
• UniProt accession no.: P18206
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... VCL(7414)

Description

General description

117kDa

Immunogen

Human Vinculin

Application

Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Vinculin

Physical form

Format: Purified

PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA

Protein A chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable