All Photos(4)

05-389

Sigma-Aldrich

Anti-Rac1 Antibody, clone 23A8

clone 23A8, Upstate®, from mouse

eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

23A8, monoclonal

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

ambient

Gene Information

human ... RAC1(5879)

Related Categories

General description

Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.

Specificity

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

Immunogen

Recombinant protein containing the full length human Rac

Application

Research Sub Category
Cytoskeletal Signaling

G-proteins
Research Category
Signaling
This Anti-Rac1 Antibody, clone 23A8 has been published in more than 40 citations and validated for use in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Rac1 protein.

Quality

routinely evaluated by immunoblot on rat brain microsomal protein preparation

Target description

21 kDa

Physical form

Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors attenuate beta-amyloid-induced microglial inflammatory responses.
Cordle, A; Landreth, G
The Journal of Neuroscience null
Enikö A Kramár et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(41), 12982-12993 (2009-10-16)
Estrogen, in addition to its genomic effects in brain, causes rapid and reversible changes to synaptic operations. We report here that these acute actions are due to selective activation of an actin-signaling cascade normally used in the production of long-term...
Expression of NADPH oxidase in rabbit corneal epithelial and stromal cells in culture.
O'Brien, WJ; Krema, C; Heimann, T; Zhao, H
Investigative Ophthalmology & Visual Science null
Androgen-stimulated DNA synthesis and cytoskeletal changes in fibroblasts by a nontranscriptional receptor action
Castoria, G., et al
The Journal of cell biology, 161, 547-556 (2003)
H N Kovacic et al.
The Journal of biological chemistry, 276(49), 45856-45861 (2001-10-05)
Rac1 has been shown to activate a NADPH oxidase complex producing superoxide anions in a variety of mammalian cell types. We evaluated the impact of Rac1-induced reactive oxygen species production on the turnover of Rac1 itself in human aortic endothelial...

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