MilliporeSigma
All Photos(5)

Documents

05-636

Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

clone JBW301, Upstate®, from mouse

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
H2AXS139P, Histone H2A.X (phospho S139)
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

affinity purified immunoglobulin

clone

JBW301, monoclonal

species reactivity

vertebrates

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer139)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
16-202A05-636-I16-193
antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

species reactivity

vertebrates

species reactivity

human

species reactivity

human, rat, mouse

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

-

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

Upstate®

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Recognizes Histone H2A.X phosphorylated at Ser139.

Immunogen

peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X

Application

Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.

Target description

17 kDa

Linkage

Replaces: MABE205

Physical form

Format: Purified
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Protein G Purified

Storage and Stability

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Analysis Note

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Multiple roles for MRE11 at uncapped telomeres.
Deng, Y; Guo, X; Ferguson, DO; Chang, S
Nature null
Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells.
Brugat, T; Nguyen-Khac, F; Grelier, A; Merle-Beral, H; Delic, J
Blood null
FEN1 contributes to telomere stability in ALT-positive tumor cells.
Saharia, A; Stewart, SA
Oncogene null
Effect of Ku80 deficiency on mutation frequencies and spectra at a LacZ reporter locus in mouse tissues and cells.
Busuttil, RA; Mu?oz, DP; Garcia, AM; Rodier, F; Kim, WH; Suh, Y; Hasty, P; Campisi, J; Vijg, J
Testing null
Unsuitability of lymphoblastoid cell lines as surrogate of cryopreserved isolated lymphocytes for the analysis of DNA double-strand break repair activity.
Zijno, Andrea, et al.
Mutation Research, 684, 98-105 (2010)

Articles

Antibody Generation

Leverage the inherent immune ability of an animal to generate antibodies that bind to specific molecules.

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service