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05-807

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Anti-phospho-CREB (Ser133) Antibody, clone 634-2

clone 634-2, Upstate®, from mouse

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Synonym(s):
active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

634-2, monoclonal

species reactivity

rat, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pSer133)

Gene Information

human ... CREB1(1385)

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05-66706-519-AF64706-519-AF488
antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

634-2, monoclonal

clone

10E9, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, mouse

species reactivity

canine, rat, mouse, human

species reactivity

mouse, rat, human

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

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General description

CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

Specificity

Broad species cross-reactivity expected, based on sequence homology.
Phospho-CREB (Ser133).

Immunogen

A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Epitope: Ser133

Application

Beadlyte Specificity Assay:
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence

Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.

Quality

Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.

Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).

Target description

43 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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