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06-1036

Sigma-Aldrich

Anti-March5 Antibody

serum, from rabbit

Synonym(s):
membrane-associated ring finger (C3HC4) 5, Membrane-associated RING finger protein 5, RING finger protein 153, E3 ubiquitin-protein ligase MARCH5, Mitochondrial ubiquitin ligase, Membrane-associated RING-CH protein V
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

application(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Membrane-associated RING-CH protein 5 (March5), also known as NKP66, is thought to be a mitochondrial ubiquitin ligase that is localized on the mitochondrial membrane. March5 has a Plant Homeo-Domain (PHD) motif that is essential for E3 ubiquitin ligase action. MARCH 5 is also thought to have four transmembrane domains. March5 has been associated with mitochondrial fission and division. March5 also seems to have a defensive role against mitochondrial dysfunction by interacting with mutant superoxide dismutase 1 (mSOD1) and preventing mSOD1 from causing damage. mSOD1 damage has been linked to the onset and progression of amyotrophic lateral sclerosis.

Immunogen

KLH-conjugated linear peptide corresponding to human March5.
.

Application

Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Research Category
Epigenetics & Nuclear Function
Anti-March5 Antibody is a Rabbit Polyclonal Antibody for detection of March5 also known as Membrane-associated RING finger protein 5 & has been validated in WB, ICC.
Immunocytochemistry Analysis: A representative lot of this antibody was used by an independent laboratory in IC. (Korfali, N., et al. (2010). Mol Cell Proteomics. 9:2571-2585.)

Quality

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected March5 on 10 µg of HL-60 cell lysate.

Target description

~52 kDa observed

Physical form

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HL-60 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

MARCH5-dependent degradation of MCL1/NOXA complexes defines susceptibility to antimitotic drug treatment.
Haschka, et al.
Cell Death and Differentiation, 27, 2297-2312 (2021)
Seiji Arai et al.
eLife, 9 (2020-06-03)
MCL1 has critical antiapoptotic functions and its levels are tightly regulated by ubiquitylation and degradation, but mechanisms that drive this degradation, particularly in solid tumors, remain to be established. We show here in prostate cancer cells that increased NOXA, mediated...
The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture.
Korfali, N; Wilkie, GS; Swanson, SK; Srsen, V; Batrakou, DG; Fairley, EA; Malik et al.
Molecular and Cellular Proteomics null
Lilian Phu et al.
Molecular cell, 77(5), 1107-1123 (2020-03-07)
Mitochondria import nearly their entire proteome from the cytoplasm by translocating precursor proteins through the translocase of the outer membrane (TOM) complex. Here, we show dynamic regulation of mitochondrial import by the ubiquitin system. Acute pharmacological inhibition or genetic ablation...
Alban Ordureau et al.
Molecular cell, 77(5), 1124-1142 (2020-03-07)
The ubiquitin ligase Parkin, protein kinase PINK1, USP30 deubiquitylase, and p97 segregase function together to regulate turnover of damaged mitochondria via mitophagy, but our mechanistic understanding in neurons is limited. Here, we combine induced neurons (iNeurons) derived from embryonic stem...

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