06-1085-I

Anti-IL-7Ra (CD127), extracellular Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD127
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702

Properties

• Product Name: Anti-IL-7Ra (CD127), extracellular Antibody, from rabbit, purified by affinity chromatography
• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): flow cytometry: suitable; western blot: suitable
• NCBI accession no.: NP_002176
• UniProt accession no.: P16871
• shipped in: wet ice
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... IL7R(3575)

Description

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-7Ra (CD127) in 10 µg of K562 cell lysate.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-IL-7Ra (CD127), extracellular Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of IL-7Ra (CD127), extracellular.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected IL-7Ra (CD127) in 10 µg of human spleen tissue lysate.
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected IL-7Ra (CD127) in 1X10E6 human peripheral blood mononuclear cells (PBMCs).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The R34-34 antibody is specific for human CD127, a cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity. The R34-34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM), whereas certain populations of Foxp3+ /CD25+ T cells lacking CD127 have been associated with impaired suppressive function (Bikker et al. 2012. Rheumatology, 51: 996-1005)

~70 kDa observed. This protien is highly and variably glycosylated, and may vary from the calculated molecular weight of ~52kDa (Vranjkovi, A., et al., International Immunology, Vol. 19, No. 12, pp. 1329–1339). Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human IL-7Ra (CD127).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable