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06-1295

Sigma-Aldrich

Anti-CDT1 Antibody

serum, from rabbit

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Synonym(s):
DNA replication factor, Double parked homolog, Double parked, Drosophila, homolog of, chromatin licensing and DNA replication factor 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDT1(81620)
mouse ... Cdt1(67177)
rat ... Cdt1(292071)

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vibrant-m

06-1295

Anti-CDT1 Antibody

vibrant-m

ABS500

Anti-CDT2 Antibody

vibrant-m

ABT180

Anti-CDCP1 Antibody

vibrant-m

ABT66

Anti-CADM1 Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

serum

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

mouse, rat, human

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human, mouse, rat

UniProt accession no.

Q9H211

UniProt accession no.

Q9NZJ0

UniProt accession no.

Q9H5V8

UniProt accession no.

Q9BY67

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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General description

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

Specificity

This antibody recognizes CDT1.

Immunogen

KLH-conjugated linear peptide corresponding to human CDT1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog.
Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates.

Quality

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.

Target description

~60 kDa

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Suzanne A Hartford et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17702-17707 (2011-10-12)
Effective DNA replication is critical to the health and reproductive success of organisms. The six MCM2-7 proteins, which form the replicative helicase, are essential for high-fidelity replication of the genome. Many eukaryotes have a divergent paralog, MCM9, that was reported
Chantal M Maghames et al.
Nature communications, 9(1), 4376-4376 (2018-10-24)
Spatial management of stress-induced protein aggregation is an integral part of the proteostasis network. Protein modification by the ubiquitin-like molecule NEDD8 increases upon proteotoxic stress and it is characterised by the formation of hybrid NEDD8/ubiquitin conjugates. However, the biological significance
Julia I Toth et al.
Cell reports, 1(4), 309-316 (2012-07-27)
Inhibition of NEDD8-activating enzyme (NAE) has emerged as a highly promising approach to treat cancer through the adenosine sulfamate analog MLN4924. Here, we show that selective pressure results in HCT116 colorectal carcinoma cells with decreased MLN4924 sensitivity and identify a
Richard O Adeyemi et al.
PLoS pathogens, 10(4), e1004055-e1004055 (2014-04-05)
Infection by the autonomous parvovirus minute virus of mice (MVM) induces a vigorous DNA damage response in host cells which it utilizes for its efficient replication. Although p53 remains activated, p21 protein levels remain low throughout the course of infection.

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