• 061295
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Anti-CDT1 Antibody

serum, from rabbit

DNA replication factor, Double parked homolog, Double parked, Drosophila, homolog of, chromatin licensing and DNA replication factor 1

biological source


Quality Level

antibody form


antibody product type

primary antibodies



species reactivity

mouse, rat, human


western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CDT1(81620)
mouse ... Cdt1(67177)
rat ... Cdt1(292071)

General description

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).


This antibody recognizes CDT1.


KLH-conjugated linear peptide corresponding to human CDT1.


Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog.
Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates.


Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.

Target description

~60 kDa

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

HeLa cell lysate


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids



Certificate of Analysis

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Certificate of Quality

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