mRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain (CTD) and functions by adding an inverted guanylyl group (also known as a ‘cap’) to the 5’ end. mRNA capping enzyme also has triphosphatase and guanylyltransferase activity. The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex. mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.
GST-tagged recombinant protein corresponding to human mRNA-capping enzyme.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected mRNA-capping enzyme in NIH/3T3, A431, and HeLa cells.
Research Sub Category
Epigenetics & Nuclear Function
Use Anti-mRNA-capping enzyme Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect mRNA-capping enzyme also known as mRNA-capping enzyme, HCAP1, HCE.
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 1:5,000 dilution of this antibody detected mRNA-capping enzyme on 10 µg of HeLa nuclear extract.
~66 kDa observed
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
HeLa nuclear extract
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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