06-1354

Sigma-Aldrich

Anti-mRNA-capping enzyme Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
mRNA-capping enzyme, HCAP1, HCE, Polynucleotide 5′-triphosphatase, mRNA 5′-triphosphatase, Tpase, mRNA guanylyltransferase, GTP--RNA guanylyltransferase, GTase
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

application(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... RNGTT(8732)

General description

mRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain (CTD) and functions by adding an inverted guanylyl group (also known as a ‘cap’) to the 5’ end. mRNA capping enzyme also has triphosphatase and guanylyltransferase activity. The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex. mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.

Immunogen

GST-tagged recombinant protein corresponding to human mRNA-capping enzyme.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected mRNA-capping enzyme in NIH/3T3, A431, and HeLa cells.
Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Use Anti-mRNA-capping enzyme Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect mRNA-capping enzyme also known as mRNA-capping enzyme, HCAP1, HCE.

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1:5,000 dilution of this antibody detected mRNA-capping enzyme on 10 µg of HeLa nuclear extract.

Target description

~66 kDa observed

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

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