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06-371

Sigma-Aldrich

Rabbit Anti-Mouse IgG Antibody

Upstate®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

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This Item
AP160M-M04-1643AP160F
vibrant-m

06-371

Rabbit Anti-Mouse IgG Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunofluorescence: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable

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Specificity

mouse IgG

Immunogen

Highly purified whole mouse IgG (heavy and light chains)

Application

Rabbit anti-Mouse IgG Antibody detects level of Mouse IgG & has been published & validated for use in IP.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Quality

routinely evaluated by immunoprecipitating mouse IgG then blotting with anti-Fak (Catalog #05-182)

Target description

50-60kDa

Physical form

Format: Purified
Protein A chromatography
of 0.02M Potassium Phosphate, pH 7.2, 0.15M NaCl, with 0.01% sodium azide

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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M Yamamoto et al.
The Journal of biological chemistry, 272(19), 12492-12494 (1997-05-09)
The DF3/MUC1 mucin-like glycoprotein is aberrantly overexpressed in human breast carcinomas. The functional role of DF3 is unknown. The present studies demonstrate that DF3 associates with beta-catenin. Similar findings have been obtained for gamma-catenin but not alpha-catenin. DF3, like E-cadherin
Thomas A Milne et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(41), 14765-14770 (2005-10-04)
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Arthritis and rheumatism, 56(3), 892-904 (2007-03-01)
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