06-600

Sigma-Aldrich

Anti-NR2B Antibody

Upstate®, from rabbit

Synonym(s):
N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 2B, N-methyl-D-aspartate receptor subunit 3, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), monkey (based on 100% sequence homology), human (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

manufacturer/tradename

Upstate®

application(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease, epilepsy and ischemic neuronal cell death.

Specificity

NR2B; the immungen sequence is identical in mouse, rat, human, bovine, dog and chimp NR2B. Human NR2B is sometimes referred to as hNR3 in the literature.

Immunogen

a.a. 1437-1456 of mature mouse NR2B or 1463-1482 of NR2B precursor; signal is a.a. residues 1-26 [KFNGSSNG HVYEKLSSIESDV]. This sequence is identical to a.a. 1437-1456 of mature rat NR2B and a.a. 1465-1484 of human NR3, containing a N-terminal lysine.

Application

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
Research Category
Neuroscience
Detect NR2B using this Anti-NR2B Antibody validated for use in IC, IP & WB.

Quality

Routinely evaluated by Western Blot on rat brain microsomal preparation (Catalog # 12-144).

Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected NR2B (~180 kDa) in rat brain microsomal preparation (Catalog # 12-144).

Target description

180 kDa

Physical form

Format: Purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, and 0.05% sodium azide. Frozen solution.
Protein A purified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Mouse brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Duncan Sinclair et al.
Journal of neurodevelopmental disorders, 8, 14-14 (2016-05-03)
Neurodevelopmental disorders such as autism spectrum disorders and schizophrenia differentially impact males and females and are highly heritable. The ways in which sex and genetic vulnerability influence the pathogenesis of these disorders are not clearly understood. The n-methyl-d-aspartate (NMDA) receptor...
Perturbing PSD-95 interactions with NR2B-subtype receptors attenuates spinal nociceptive plasticity and neuropathic pain.
D'Mello, R; Marchand, F; Pezet, S; McMahon, SB; Dickenson, AH
Molecular Therapy null
Tianyun Zhao et al.
Scientific reports, 6, 26865-26865 (2016-05-27)
Ketamine is commonly used for anesthesia and as a recreational drug. In pregnant users, a potential neurotoxicity in offspring has been noted. Our previous work demonstrated that ketamine exposure of pregnant rats induces affective disorders and cognitive impairments in offspring....
Peripheral N-methyl-d-aspartate receptors contribute to mechanical hypersensitivity in a rat model of inflammatory temporomandibular joint pain.
Ivanusic, JJ; Beaini, D; Hatch, RJ; Staikopoulos, V; Sessle, BJ; Jennings, EA
European Journal of Pain null
Polyglutamine-modulated striatal calpain activity in YAC transgenic huntington disease mouse model: impact on NMDA receptor function and toxicity.
Cowan, CM; Fan, MM; Fan, J; Shehadeh, J; Zhang, LY; Graham, RK; Hayden, MR; Raymond, LA
The Journal of Neuroscience null
Articles
The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.
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