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06-680

Sigma-Aldrich

Anti-Androgen Receptor Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

ChIP: suitable (ChIP-seq)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

bovine ... Ar(280675)
dog ... Ar(403588)
human ... AR(367)
mouse ... Ar(11835)
rat ... Ar(24208)

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This Item
06-680-AF647A985306-935
Anti-Androgen Receptor Antibody from rabbit, purified by affinity chromatography

06-680

Anti-Androgen Receptor Antibody

Anti-Estrogen Receptor α Antibody from rabbit, purified by affinity chromatography

06-935

Anti-Estrogen Receptor α Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, human

species reactivity

rat, human

species reactivity

rat, human

species reactivity

human

technique(s)

ChIP: suitable (ChIP-seq), flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections, immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg), microarray: suitable, western blot: 1-2 μg/mL using whole extract of rat brain

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_036634

NCBI accession no.

NP_036634

NCBI accession no.

-

NCBI accession no.

NP_036821

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General description

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Immunogen

Epitope: This antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.
KLH-conjugated linear peptide corresponding to the N-terminus of rat Androgen Receptor.

Application

Anti-Androgen Receptor Antibody, PG-21 is a Rabbit Polyclonal Antibody for detection of Androgen Receptor also known as Dihydrotestosterone Receptor & has been tested in ICC, IHC, IP & WB.
Immunoprecipitation Analysis: 34 µL from a representative lot immunoprecipitated Androgen Receptor from 1000 µg of LNCaP cell lysate.

Flow Cytometry Analysis: A 1:300 dilution from a representative lot detected Androgen Receptor in 0.2X10E6 MCF-7 cells.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Li, X., et al. (2011). PLoS One. 6(9):e25040.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6):R1036-1050.).

Chromatin Immunoprecipitaton-Sequence Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Androgen Receptor in LNCaP cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in 10 µg of LNCaP cell lysate.

Target description

~110 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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The progestational and androgenic properties of medroxyprogesterone acetate: gene regulatory overlap with dihydrotestosterone in breast cancer cells.
Ghatge, Radhika P, et al.
Breast Cancer Research, 7, R1036-R1050 (2005)
Human prostate side population cells demonstrate stem cell properties in recombination with urogenital sinus mesenchyme.
Foster, BA; Gangavarapu, KJ; Mathew, G; Azabdaftari, G; Morrison, CD; Miller, A; Huss, WJ
Testing null
Hemostatic gelatin sponge is a superior matrix to matrigel for establishment of LNCaP human prostate cancer in nude mice.
Cui, L; Chen, P; Tan, Z; Li, W; Dong, Z
Prostate null
Neonatal MeCP2 is important for the organization of sex differences in vasopressin expression.
Forbes-Lorman, RM; Rautio, JJ; Kurian, JR; Auger, AP; Auger, CJ
Epigenetics null
Androgen influence on prefrontal dopamine systems in adult male rats: localization of cognate intracellular receptors in medial prefrontal projections to the ventral tegmental area and effects of gonadectomy and hormone replacement on glutamate-stimulated extracellular dopamine level.
Aubele, T; Kritzer, MF
Cerebral Cortex (1991)

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