MilliporeSigma
All Photos(8)

Documents

06-866

Sigma-Aldrich

Anti-acetyl-Histone H4 Antibody

serum, Upstate®

Sign Into View Organizational & Contract Pricing

Synonym(s):
H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Tetrahymena sp., human, eukaryotes

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

acetylation (Lys5,Lys8,Lys 12,Lys16)

Gene Information

human ... H4C1(8359)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
06-59906-59807-327
antibody form

serum

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

Tetrahymena sp., human, eukaryotes

species reactivity

mouse, rat, human

species reactivity

Tetrahymena sp., human

species reactivity

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

ChIP: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, western blot: suitable

technique(s)

ChIP: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, western blot: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
Other species not tested, but expected to cross-react since Histone H4 is well conserved.

Immunogen

Epitope: a.a. 2-19
KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.

Application

Chromatin Immunoprecipitation:
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.

Quality

Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Target description

10 kDa

Physical form

Unpurified
Whole antiserum containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.
Sharma, D; Fondell, JD
Proceedings of the National Academy of Sciences of the USA null
The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation.
Caretti, G; Di Padova, M; Micales, B; Lyons, GE; Sartorelli, V
Genes & Development null
E C Forsberg et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(26), 14494-14499 (2000-12-20)
We have defined the histone acetylation pattern of the endogenous murine beta-globin domain, which contains the erythroidspecific beta-globin genes. The beta-globin locus control region (LCR) and transcriptionally active promoters were enriched in acetylated histones in fetal liver relative to fetal
Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells.
Zaidi, SK; Young, DW; Pockwinse, SM; Javed, A; Lian, JB; Stein, JL; van Wijnen, AJ; Stein, GS
Proceedings of the National Academy of Sciences of the USA null
Promoter-specific p53-dependent histone acetylation following DNA damage.
Kaeser, MD; Iggo, RD
Oncogene null

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service