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06-942-S

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size

Upstate®, from rabbit

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Synonym(s):
H3K9Ac, Histone H3 (acetyl K9), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, eukaryotes, mouse, rat

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

acetylation (Lys9)

Gene Information

human ... HIST1H3F(8968)

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This Item
07-450-SABE101-SABE18-S
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

human, eukaryotes, mouse, rat

species reactivity

mouse, chicken, human

species reactivity

human

species reactivity

mouse, chicken, human

technique(s)

ChIP: suitable (ChIP-seq), western blot: suitable, dot blot: suitable

technique(s)

dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable

technique(s)

dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, multiplexing: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

This peptide sequence is identical in a wide range of animal and plant species.

Immunogen

KLH-conjugated, synthetic peptide (ARTKQTA RAcKSTG-C) corresponding to amino acids 1-12 of Histone H3.

Application

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9 (H3K9ac). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ChIP, ChIP-seq, FC. Small pack size is ideal for screening applications.
Western Blot Analysis:
A431, C2C12 lysate, HEK293 lysate, Hela
lysate, HepG2 lysate , Jurkat lysate , L6
lysate, and NIH/3T3 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ANTI-ACETYL HIS H3 (1:500 dilution of a representative lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 06-942-S, Anti-acetyl-Histone H3 (Lys9) at a (1:500) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis:
0.5 μg/mL of a previous lot detected 50 ng of mono-acetylated peptide corresponding to position 9 in the N-terminal sequence of Histone H3 (Catalog # 12-358), but not the monoacetylated peptide corresponding to acetyl-lysine at position 14 (Catalog # 12-359) or unacetylated Histone H3 (Catalog # 12-357).

Immunoprecipitation:
Not determined.

Immunocytochemistry:
Not determined.

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ANTI-ACETYL HIS H3 on 10 μg of HEK293 lysates.

Target description

Approx. 17 kDa

Physical form

Format: Purified
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Analysis Note

Control
TSA-treated HeLa and NIH/3T3 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kumari Sweta et al.
Molecular microbiology, 116(1), 80-96 (2021-02-04)
Eleven-nineteen lysine-rich leukemia (ELL) is a eukaryotic RNA polymerase II transcription elongation factor. In Schizosaccharomyces pombe, it is important for survival under genotoxic stress conditions. However, the molecular basis underlying this function of ELL in S. pombe is yet to
Graeme J Gowans et al.
Molecular cell, 76(6), 909-921 (2019-11-05)
Metabolic signaling to chromatin often underlies how adaptive transcriptional responses are controlled. While intermediary metabolites serve as co-factors for histone-modifying enzymes during metabolic flux, how these modifications contribute to transcriptional responses is poorly understood. Here, we utilize the highly synchronized
Eunice Wm Chin et al.
JCI insight, 7(14) (2022-07-23)
Synaptic dysfunction is a manifestation of several neurobehavioral and neurological disorders. A major therapeutic challenge lies in uncovering the upstream regulatory factors controlling synaptic processes. Plant homeodomain (PHD) finger proteins are epigenetic readers whose dysfunctions are implicated in neurological disorders.

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