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MilliporeSigma

07-130

Anti-Ezrin Antibody

Upstate®, from rabbit

Synonym(s):

Anti-CVIL, Anti-CVL, Anti-HEL-S-105, Anti-VIL2

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200 μG

$490.00

$490.00


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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Product Name

Anti-Ezrin Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

mouse ... Ezr(22350)

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This Item
SAB4503077SAB4503079E1281
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

mouse, human

species reactivity

mouse, rat, human

species reactivity

mouse, human, rat

species reactivity

human, rat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:40000, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: 20-40 μg/mL, immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells, indirect immunofluorescence: 10-20 μg/mL, western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
routinely evaluated by immunoblot on RIPA lysates from A431 cells or a mouse brain lysate

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Ezrin Antibody is validated for use in WB, IH for the detection of Ezrin.

Biochem/physiol Actions

Ezrin

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

81kDa
Ezrin is also referred to as Villin-2 and Cytovillin

Immunogen

peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG)

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A chromatography

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Storage Class

10 - Combustible liquids

wgk

WGK 1


Certificates of Analysis (COA)

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Changxue Lu et al.
Steroids, 76(9), 885-891 (2011-04-09)
Thyroid hormone receptors (TRs) mediate the critical activities of the thyroid hormone (T3) in growth, development, and differentiation. Decreased expression and/or somatic mutations of TRs have been shown to be associated with several types of human cancers including liver, breast
Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement
Potter, D. A., et al
The Journal of Biological Chemistry, 278, 30403-30412 (2003)
Ana Cicvaric et al.
Annals of medicine, 48(8), 652-668 (2016-08-26)
Podoplanin is a cell-surface glycoprotein constitutively expressed in the brain and implicated in human brain tumorigenesis. The intrinsic function of podoplanin in brain neurons remains however uncharacterized. Using an established podoplanin-knockout mouse model and electrophysiological, biochemical, and behavioral approaches, we
Proteomics analysis of the ezrin interactome in B cells reveals a novel association with Myo18a?.
Ken Matsui,Neetha Parameswaran,Nayer Bagheri,Belinda Willard,Neetu Gupta
Journal of Proteome Research null
Teresita N J Flores-Téllez et al.
PloS one, 10(7), e0131605-e0131605 (2015-07-03)
Prognostic markers are important for predicting the progression and staging of hepatocellular carcinoma (HCC). Ezrin (EZR) and Podocalyxin (PODXL) are proteins associated with invasion, migration and poor prognosis in various types of cancer. Recently, it has been observed that chloride

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