07-1383

Sigma-Aldrich

Anti-CDT1 Antibody

serum, from rabbit

Synonym(s):
DNA replication factor , Double parked homolog , Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

application(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

Specificity

This antibody recognizes the Cdt1.

Immunogen

Epitope: C-terminal half
Recombinant protein including amino acids 238-546 of human CDT1.

Application

Research Sub Category
Cell Cycle, DNA Replication & Repair
An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).
Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.
Research Category
Signaling

Quality

Evaluated by western blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.

Target description

65 kDa

Physical form

Unpurified rabbit serum containing 0.05% NaN3.

Storage and Stability

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Yue Yuan et al.
The Journal of reproduction and development, 63(5), 481-488 (2017-07-12)
Geminin plays a critical role in cell cycle regulation by regulating DNA replication and serves as a transcriptional molecular switch that directs cell fate decisions. Spermatogonia lacking Geminin disappear during the initial wave of mitotic proliferation, while geminin is not...
Xue-Shan Ma et al.
Molecular biology of the cell, 27(5), 768-775 (2016-01-15)
Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in...
Sang-Wook Yoon et al.
Stem cells and development, 23(22), 2700-2711 (2014-07-06)
Homologous recombination (HR) maintains genomic integrity against DNA replication stress and deleterious lesions, such as double-strand breaks (DSBs). Rad51 recombinase is critical for HR events that mediate the exchange of genetic information between parental chromosomes in eukaryotes. Additionally, Rad51 and...
Human papillomavirus E7 induces rereplication in response to DNA damage.
Fan, X; Liu, Y; Heilman, SA; Chen, JJ
Journal of virology null

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