MilliporeSigma
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07-1383

Sigma-Aldrich

Anti-CDT1 Antibody

serum, from rabbit

Synonym(s):
DNA replication factor , Double parked homolog , Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CDT1(81620)
mouse ... Cdt1(67177)
rat ... Cdt1(292071)

General description

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

Specificity

This antibody recognizes the Cdt1.

Immunogen

Epitope: C-terminal half
Recombinant protein including amino acids 238-546 of human CDT1.

Application

An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).
Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.
Research Category
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by western blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.

Target description

65 kDa

Physical form

Unpurified rabbit serum containing 0.05% NaN3.

Storage and Stability

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Quality

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