07-1464
Anti-Rac1 Antibody
from rabbit
Synonym(s):
Ras-related C3 Botulinum Toxin Substrate 1, p21-Rac1, Ras-like Protein TC25
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100 μG
$519.00
$519.00
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A recombinant, preservative-free antibody is available for your target. Try ZMS1023
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
6
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biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RAC1(5879)
1 of 4
This Item | |||
|---|---|---|---|
| clone polyclonal | clone polyclonal | clone 23A8, monoclonal | clone 23A8, monoclonal |
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| species reactivity human, rat, mouse | species reactivity mouse, human | species reactivity mouse, rat, human | species reactivity rat, mouse, human |
| antibody form purified antibody | antibody form affinity purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified antibody |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| technique(s) western blot: suitable | technique(s) immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable | technique(s) immunocytochemistry: suitable | technique(s) immunocytochemistry: suitable |
General description
21 kDa
Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
Immunogen
Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).
Application
Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative
Preparation Note
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4202
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Joon-Il Jun et al.
Nature cell biology, 12(7), 676-685 (2010-06-08)
Cellular senescence is a recognized mechanism of tumour suppression; however, its contribution to other pathologies is not well understood. We show that the matricellular protein CCN1 (also known as CYR61; cysteine-rich protein 61), which is dynamically expressed at sites of
D Araiza-Olivera et al.
Oncogene, 37(7), 944-952 (2017-10-24)
Activating mutations in the RAC1 gene have recently been discovered as driver events in malignant melanoma. Expression of this gene is associated with melanocyte proliferation, and melanoma cells bearing this mutation are insensitive to BRAF inhibitors such as vemurafenib and
Jeffrey M Hord et al.
Experimental gerontology, 83, 148-157 (2016-08-19)
Age-related loss of skeletal muscle mass and function, referred to as sarcopenia, is mitigated by lifelong calorie restriction as well as exercise. In aged skeletal muscle fibers there is compromised integrity of the cell membrane that may contribute to sarcopenia.
Jeffrey M Hord et al.
Physiological reports, 9(1), e14606-e14606 (2021-01-06)
Reduced mechanical loading results in atrophy of skeletal muscle fibers. Increased reactive oxygen species (ROS) are causal in sarcolemmal dislocation of nNOS and FoxO3a activation. The Nox2 isoform of NADPH oxidase and mitochondria release ROS during disuse in skeletal muscle.
Nicolas Bery et al.
Cell chemical biology, 26(11), 1544-1558 (2019-09-17)
The selective downregulation of activated intracellular proteins is a key challenge in cell biology. RHO small GTPases switch between a guanosine diphosphate (GDP)-bound and a guanosine triphosphate (GTP)-bound state that drives downstream signaling. At present, no tool is available to
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