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07-1496

Sigma-Aldrich

Anti-CaM Kinase II Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Anti-CAMKA, Anti-CaMKIINalpha, Anti-CaMKIIalpha, Anti-MRD53, Anti-MRT63
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, chicken, bovine, hamster, human, rabbit, rat, guinea pig

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CAMK2A(815)

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This Item
07-138705-532WH0010645M1
antibody form

affinity purified immunoglobulin

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

6G9, monoclonal

clone

1A11, monoclonal

species reactivity

mouse, chicken, bovine, hamster, human, rabbit, rat, guinea pig

species reactivity

mouse, rat, human

species reactivity

rat, bovine

species reactivity

human

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

NCBI accession no.

NM_015981.2

NCBI accession no.

NP_742127

NCBI accession no.

NM_015981

NCBI accession no.

-

General description

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Specificity

Recognizes proteins of 50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II α subunits expressed in sf9 insect cells are also detected. As the sequences of the rat α, δ, and γ isoforms are conserved over this amino terminus region, this antibody is expected to recognize the δ, γ, and α isoforms. Antibody is not expected to cross-react with the β isoform. Proteins of unknown identity may be detected on immunoblot analysis with some lysates (40, 45 and 90 kDa).

Immunogen

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of Rat CaMK II

Application

Anti-CaM Kinase II Antibody is an antibody against CaM Kinase II for use in WB.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.

Target description

50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms.

Linkage

Replaces: AB3111

Physical form

Affinity purified
Purified rabbit polyclonal IgG in buffer containing borate buffered saline and phosphate buffered saline containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. For optimal storage, aliquot and store at -20°C or -70°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HEK293 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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R M Tombes et al.
Biochimica et biophysica acta, 1452(1), 1-11 (1999-10-19)
Over two dozen alternative splice variants of CaMK-II, the type II Ca(2+)/CaM-dependent protein kinase, are encoded from four genes (alpha, beta, gamma and delta) in mammalian cells. Isozymes of alpha and beta CaMK-II are well characterized in brain; however, an
C Rongo et al.
Nature, 402(6758), 195-199 (2000-01-26)
Synaptic connections undergo a dynamic process of stabilization or elimination during development, and this process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. The type II calcium- and calmodulin-dependent protein kinase
Y H Koh et al.
Cell, 98(3), 353-363 (1999-08-24)
Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated
DongZhu Xu et al.
Clinical science (London, England : 1979), 135(20), 2409-2422 (2021-08-14)
Oxidative stress could be a possible mechanism and a therapeutic target of atrial fibrillation (AF). However, the effects of the xanthine oxidase (XO) inhibition for AF remain to be fully elucidated. We investigated the effects of a novel XO inhibitor
F Gardoni et al.
FEBS letters, 456(3), 394-398 (1999-08-26)
Ca2+/calmodulin-dependent protein kinase II (CaMKII), a multifunctional, widely distributed enzyme, is enriched in post-synaptic densities (PSDs). Here, we demonstrate that CaMKII binds to a discrete C-terminal region of the NR2A subunit of NMDA receptors and promotes the phosphorylation of a

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