Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.
Specific for cAMP with no cross reaction to other nucleotides at concentrations to 1 mM. Reactive with cAMP fixed in tissues or acetylated cAMP. Not reactive with free, non-acetylated, cAMP in RIA or EIA assays.
2′O-succinyl cyclic AMP conjugated to BSA
Anti-cAMP Antibody detects level of cAMP & has been published & validated for use in IH(P).
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Evaluated by Immunohistochemistry in rat hippocampus and cerebellum tissue.
Immunohistochemistry Analysis: 1:100 dilution of this lot detected cAMP in rat hippocampus and cerebellum tissue.
Unpurified rabbit serum
Diluted serum presented as liquid in PBS containing no preservatives.
Storage and Stability
Maintain at -20°C in undiluted aliquots from date of shipment. Avoid repeated freeze/thaw cycles.
Rat hippocampus and cerebellum tissue
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