07-1497

Sigma-Aldrich

Anti-cAMP Antibody

serum, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

all

application(s)

immunohistochemistry: suitable (paraffin)

shipped in

wet ice

General description

Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.

Specificity

Specific for cAMP with no cross reaction to other nucleotides at concentrations to 1 mM. Reactive with cAMP fixed in tissues or acetylated cAMP. Not reactive with free, non-acetylated, cAMP in RIA or EIA assays.

Immunogen

2′O-succinyl cyclic AMP conjugated to BSA

Application

Anti-cAMP Antibody detects level of cAMP & has been published & validated for use in IH(P).
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Research Category
Signaling

Quality

Evaluated by Immunohistochemistry in rat hippocampus and cerebellum tissue.
Immunohistochemistry Analysis: 1:100 dilution of this lot detected cAMP in rat hippocampus and cerebellum tissue.

Linkage

Replaces: MABN1026

Physical form

Unpurified rabbit serum
Diluted serum presented as liquid in PBS containing no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots from date of shipment. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Rat hippocampus and cerebellum tissue

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.
Luo, Q; Kumar, P; Vickers, TJ; Sheikh, A; Lewis, WG; Rasko, DA; Sistrunk, J; Fleckenstein, JM
Infection and Immunity null
Cíntia Yuki Fukuoka et al.
PloS one, 15(8), e0236727-e0236727 (2020-08-05)
Low-power laser irradiation (LPLI) is clinically used to modulate inflammation, proliferation and apoptosis. However, its molecular mechanisms are still not fully understood. This study aimed to describe the effects of LPLI upon inflammatory, apoptotic and proliferation markers in submandibular salivary...
Robbert Havekes et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(34), 8936-8946 (2016-08-26)
Alterations in cAMP signaling are thought to contribute to neurocognitive and neuropsychiatric disorders. Members of the cAMP-specific phosphodiesterase 4 (PDE4) family, which contains >25 different isoforms, play a key role in determining spatial cAMP degradation so as to orchestrate compartmentalized...
Wei Liu et al.
The Journal of experimental medicine, 217(4) (2020-01-10)
Progressive loss of retinal ganglion cells (RGCs) leads to irreversible visual deficits in glaucoma. Here, we found that the level of cyclic AMP and the activity and expression of its mediator Epac1 were increased in retinas of two mouse models...

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