07-2129

Anti-phospho-Abi-1 (Ser225) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: monkey, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_001012768
• UniProt accession no.: Q8IZP0
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer225)
• Gene Information: human ... ABI1(10006)

Description

General description

Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.

~60 kDa observed.
55 kDa calculated.

Immunogen

Epitope: Phosphorylated Ser225

KLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).

Biochem/physiol Actions

This antibody recognizes Abi-1 phosphorylated at Ser225.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
EGF-treated COS cells untreated and treated with lambda phosphatase.

Evaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable