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Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody

Upstate®, from rabbit

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Synonym(s):
acetyl-CoA carboxylase 1, acetyl-CoA carboxylase-alpha, acetyl-Coenzyme A carboxylase alpha
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

canine, human

species reactivity (predicted by homology)

bovine (immunogen homology), chicken (immunogen homology), rat (immunogen homology), mouse (immunogen homology), dog (immunogen homology)

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

phosphorylation (pSer79)

Gene Information

human ... ACACA(31)

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clone

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clone

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species reactivity

canine, human

species reactivity

human, rat

species reactivity

mouse, human

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

-

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

General description

Acetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Specificity

Recognizes Acetyl CoA Carboxylase phosphorylated at Serine 79, MW 257 kDa.

Immunogen

Epitope: N-terminal
KLH conjugated synthetic peptide (C-HM RSSM[pS]GLHLVK) corresponding to amino acid 73-85 of rat Acetyl CoA Carboxylase.

Application

Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody is an antibody against phospho-Acetyl CoA Carboxylase (Ser79) for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blot on Lambda phosphatase-treated and untreated A375 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.

Target description

257 kDa

Linkage

Replaces: 04-1009

Physical form

Format: Purified
Protein A chromatography
Protein A purified in buffer containing in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Xiaoqing Zhu et al.
The Journal of biological chemistry, 291(33), 17020-17027 (2016-07-15)
AMP-activated protein kinase (AMPK) is a molecular energy sensor that acts to sustain cellular energy balance. Although AMPK is implicated in the regulation of a multitude of ATP-dependent cellular processes, exactly how these processes are controlled by AMPK as well
Caffeine activates preferentially alpha1-isoform of 5'AMP-activated protein kinase in rat skeletal muscle.
Egawa T, Hamada T, Ma X, Karaike K, Kameda N, Masuda S, Iwanaka N, Hayashi T
Acta Physiologica (Oxford, England) null
Modulation by peroxynitrite of Akt- and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase
Zou, M. H., et al
The Journal of Biological Chemistry, 277, 32552-32557 (2002)
Jonas M Kristensen et al.
PloS one, 8(1), e53533-e53533 (2013-01-24)
Metformin is used as an anti-diabetic drug. Metformin ameliorates insulin resistance by improving insulin sensitivity in liver and skeletal muscle. Reduced mitochondrial content has been reported in type 2 diabetic muscles and it may contribute to decreased insulin sensitivity characteristic
Andreas M Fritzen et al.
The Journal of physiology, 594(3), 745-761 (2015-11-29)
Regulation of autophagy in human muscle in many aspects differs from the majority of previous reports based on studies in cell systems and rodent muscle. An acute bout of exercise and insulin stimulation reduce human muscle autophagosome content. An acute

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