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07-354

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys18) Antibody

serum, Upstate®

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Synonym(s):
H3K18Ac, Histone H3 (acetyl K18)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

yeast, Saccharomyces cerevisiae, human

species reactivity (predicted by homology)

vertebrates (most common)

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

acetylation (Lys18)

Gene Information

human ... H3C1(8350)

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This Item
07-354-S07-35507-677-I
antibody form

serum

antibody form

serum

antibody form

serum

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

yeast, Saccharomyces cerevisiae, human

species reactivity

human, Saccharomyces cerevisiae, yeast

species reactivity

Saccharomyces cerevisiae, human, vertebrates

species reactivity

human

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-chip), dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable, dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable, dot blot: suitable, western blot: suitable

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General description

Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Recognizes Histone H3 acetylated on lysine 18.

Immunogen

KLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes

Application

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit Serum , or 2 µL of Anti-Acetyl-Histone H3 (Lys18)and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H3 (Lys18) associated DNA fragments was verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and β-globin primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

ChIP-Sequencing:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 2 μg of anti-acetyl-Histone H3 (Lys18) antibody (cat# 07-354), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of ten million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files.

Western Blot Analysis:
Representative lot data.
Recombinant histone H3 (lane 1, Catalog # 14-494) and acid extracts from sodium butyrate treated (lane 2) and untreated (lane 3) HeLa cells (Catalog # 17-305) were probed with anti acetyl- Histone H3 (Lys18) (1:10,000 dilution).
Arrow indicates acetyl histone H3 (Lys18) (17 kDa).

Dot Blot:
Representative lot data.
40 ng and 4ng amounts of histone peptides with various modifications (see table 1) were transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys18) antibody (1:5000 dilution). Proteins were visualized using a goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Image from a 60 second exposure is shown.
Use Anti-acetyl-Histone H3 (Lys18) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB to detect acetyl-Histone H3 (Lys18) also known as H3K18Ac, Histone H3 (acetyl K18).

Quality

routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells

Target description

17 kDa

Linkage

Replaces: 04-1107

Physical form

100 μLof rabbit serum containing 0.05% sodium azide and 30% glycerol.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Inducible activation of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia and regenerative changes in the absence of "floxed" alleles.
Huh WJ, Mysorekar IU, Mills JC
American Journal of Physiology: Gastrointestinal and Liver Physiology null
Paola Y Bertucci et al.
Nucleic acids research, 41(12), 6072-6086 (2013-05-04)
Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of
Nucleosome competition reveals processive acetylation by the SAGA HAT module.
Ringel, AE; Cieniewicz, AM; Taverna, SD; Wolberger, C
Proceedings of the National Academy of Sciences of the USA null
H2B- and H3-specific histone deacetylases are required for DNA methylation in Neurospora crassa.
Smith, KM; Dobosy, JR; Reifsnyder, JE; Rountree, MR; Anderson, DC; Green, GR; Selker, EU
Genetics null
Jason S Patzlaff et al.
Experimental cell research, 316(13), 2123-2135 (2010-05-11)
Histone acetylation is a key modification that regulates chromatin accessibility. Here we show that treatment with butyrate or other histone deacetylase (HDAC) inhibitors does not induce histone hyperacetylation in metaphase-arrested HeLa cells. When compared to similarly treated interphase cells, acetylation

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