MilliporeSigma
All Photos(3)

Documents

07-360-S

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys27) Antibody, Trial Size

serum, Upstate®

Sign Into View Organizational & Contract Pricing

Synonym(s):
H3K27Ac, Histone H3 (acetyl K27)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

vertebrates, Saccharomyces cerevisiae, human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

acetylation (Lys27)

Gene Information

human ... HIST1H3F(8968)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
07-355-S06-942-S07-354-S
antibody form

serum

antibody form

serum

antibody form

purified immunoglobulin

antibody form

serum

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

technique(s)

ChIP: suitable (ChIP-seq), western blot: suitable, dot blot: suitable

technique(s)

ChIP: suitable, dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-chip), dot blot: suitable, western blot: suitable

species reactivity

vertebrates, Saccharomyces cerevisiae, human

species reactivity

human, yeast, Saccharomyces cerevisiae

species reactivity

human, eukaryotes, mouse, rat

species reactivity

human, Saccharomyces cerevisiae, yeast

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Recognizes Histone H3 acetylated on lysine 27.

Immunogen

Epitope: Lys27
Synthetic peptide corresponding to the sequence AR(acK)SA, in which acK corresponds to acetyl lysine 27 of yeast histone H3.

Application

ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 2 µl of anti-acetyl-Histone H3 (Lys27) antibody (cat# 07-360-S), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of fourteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 07-360 dataset showed 96% overlap with peaks identified in the ENCODE H3K27Ac BROAD Histone track for HeLa S3.
Anti-acetyl-Histone H3 (Lys27) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated at lysine 27 (H3K27ac). This antibody has dot blot verified specificity and has been validated in ChIP, WB & Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells

Target description

17 kDa

Physical form

Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, and 5mg/ml BSA before the addition of glycerol to 30%
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Acid extracted proteins from HeLa cells treated with sodium butyrate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Hazem E Hassan et al.
Epigenetics, 11(10), 740-749 (2016-09-03)
Curcumin and its analogs exhibited antileukemic activity either as single agent or in combination therapy. Dimethoxycurcumin (DMC) is a more metabolically stable curcumin analog that was shown to induce the expression of promoter-methylated genes without reversing DNA methylation. Accordingly, co-treatment

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service