MilliporeSigma
All Photos(1)

Documents

07-412

Sigma-Aldrich

Anti-dimethyl-Arginine Antibody, symmetric (SYM10)

serum, Upstate®

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

dimethylation (Arg)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
07-41307-41409-814
antibody form

serum

antibody form

purified antibody

antibody form

serum

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).
Does not recognize non-methylated peptides, peptides containing asymmetrically dimethylated arginines, or a peptide containing a single symmetric dimethylarginine.

Specificity

Recognizes proteins that contain multiple symmetrically dimethylated arginines, including p80-Coilin and the Sm protein B, B′ and D.

Immunogen

peptide containing four symmetrical dimethyl-arginine-glycine repeats

Application

Anti-dimethyl-Arginine Antibody, symmetric (SYM10) is a high quality Rabbit Polyclonal Antibody for the detection of dimethyl-Arginine & has been validated in ICC, IP & WB.
Research Category
Signaling
Research Sub Category
Chromatin Biology

Quality

routinely evaluated by immunoblot in RIPA lysates from Jurkat cells

Target description

varies

Physical form

Whole rabbit antiserum containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Lindsay M Webb et al.
Frontiers in immunology, 10, 524-524 (2019-04-04)
Multiple sclerosis is an autoimmune disease of the central nervous system (CNS) mediated by CD4+ T cells and modeled via experimental autoimmune encephalomyelitis (EAE). Inhibition of PRMT5, the major Type II arginine methyltransferase, suppresses pathogenic T cell responses and EAE.
Hironari Tamiya et al.
The Journal of clinical investigation, 128(1), 517-530 (2017-12-12)
SHARPIN, an adaptor for the linear ubiquitin chain assembly complex (LUBAC), plays important roles in NF-κB signaling and inflammation. Here, we have demonstrated a LUBAC-independent role for SHARPIN in regulating melanoma growth. We observed that SHARPIN interacted with PRMT5, a
Madhumitha Rengasamy et al.
Nucleic acids research, 45(19), 11106-11120 (2017-10-05)
We observed overexpression and increased intra-nuclear accumulation of the PRMT5/WDR77 in breast cancer cell lines relative to immortalized breast epithelial cells. Utilizing mass spectrometry and biochemistry approaches we identified the Zn-finger protein ZNF326, as a novel interaction partner and substrate
Arginine methylation mediated by the Arabidopsis homolog of PRMT5 is essential for proper pre-mRNA splicing.
Deng, X; Gu, L; Liu, C; Lu, T; Lu, F; Lu, Z; Cui, P; Pei, Y; Wang, B; Hu, S; Cao, X
Proceedings of the National Academy of Sciences of the USA null
Joris Paris et al.
Retrovirology, 15(1), 48-48 (2018-07-13)
Nuclear localization of Gag is a property shared by many retroviruses and retrotransposons. The importance of this stage for retroviral replication is still unknown, but studies on the Rous Sarcoma virus indicate that Gag might select the viral RNA genome

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service