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07-448

Sigma-Aldrich

Anti-monomethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Synonym(s):
H3K27me1, Histone H3 (mono methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

manufacturer/tradename

Upstate®

application(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.
Broad species cross-reactivity expected, based on sequence homology.

Immunogen

Epitope: Lys27
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Application

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-monomethyl-Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody to detect Histone H3 monomethylated at lysine 27. This purified Ab, also known as Anti-H3K27me1 is specificity verified by dot blot (DB), published in peer reviewed journals and validated in ICC, WB, ChIP, ChIP-seq.

Quality

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Target description

17 kDa

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Quantitative mass spectrometry of histones H3.2 and H3.3 in Suz12-deficient mouse embryonic stem cells reveals distinct, dynamic post-translational modifications at Lys-27 and Lys-36.
Jung, HR; Pasini, D; Helin, K; Jensen, ON
Molecular and Cellular Proteomics null
Heterochromatic genes undergo epigenetic changes and escape silencing in immunodeficiency, centromeric instability, facial anomalies (ICF) syndrome.
Brun, ME; Lana, E; Rivals, I; Lefranc, G; Sarda, P; Claustres, M; Megarbane, A; De Sario, A
Testing null
Pedro Crevillén et al.
Nature, 515(7528), 587-590 (2014-09-16)
The reprogramming of epigenetic states in gametes and embryos is essential for correct development in plants and mammals. In plants, the germ line arises from somatic tissues of the flower, necessitating the erasure of chromatin modifications that have accumulated at...
Mathieu Lupien et al.
Cell, 132(6), 958-970 (2008-03-25)
Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through...
Generation and characterization of methyl-lysine histone antibodies
Perez-Burgos, L., et al
Test, 376, 234-254 (2004)

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