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07-593

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys9/18) Antibody

serum, Upstate®

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Synonym(s):
H3K9/18Ac, Histone H3 (acetyl K9/18)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, Saccharomyces cerevisiae, bovine, human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

Gene Information

human ... H3F3B(3021)

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This Item
07-35407-354-S07-352
Anti-acetyl-Histone H3 (Lys9/18) Antibody serum, Upstate®

Sigma-Aldrich

07-593

Anti-acetyl-Histone H3 (Lys9/18) Antibody

Anti-acetyl-Histone H3 (Lys18) Antibody serum, Upstate®

Sigma-Aldrich

07-354

Anti-acetyl-Histone H3 (Lys18) Antibody

Anti-acetyl-Histone H3 (Lys18) Antibody, Trial Size serum, Upstate®

Sigma-Aldrich

07-354-S

Anti-acetyl-Histone H3 (Lys18) Antibody, Trial Size

Anti-acetyl-Histone H3 (Lys9) Antibody serum, Upstate®

Sigma-Aldrich

07-352

Anti-acetyl-Histone H3 (Lys9) Antibody

antibody form

serum

antibody form

serum

antibody form

serum

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse, Saccharomyces cerevisiae, bovine, human

species reactivity

yeast, Saccharomyces cerevisiae, human

species reactivity

human, Saccharomyces cerevisiae, yeast

species reactivity

human, Saccharomyces cerevisiae, yeast, vertebrates

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable, multiplexing: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-chip), dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), multiplexing: suitable, western blot: suitable

General description

Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Broad species cross-reactivity expected
Recognizes Histone H3 acetylated on lysines 9 and 18.

Immunogen

peptide (ARAcKSTGGKAPRAcKQL) which corresponds to acetyl-lysine at residue 9 and 18 of human Histone H3

Application

Anti-acetyl-Histone H3 (Lys9/18) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys9/18) also known as H3K9/18Ac, Histone H3 (acetyl K9/18) & has been validated in ChIP, WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

routinely evaluated by immunoblot on acid extracts from sodium butyrate treated Hela cells (Catalog #17-305)

Target description

17 kDa

Physical form

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Acid extracts from sodium butyrate treated HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Michal Mikula et al.
International journal of molecular medicine, 34(6), 1647-1654 (2014-10-17)
Obesity contributes to the development of non-alcoholic fatty liver disease (NAFLD), which is characterized by the upregulated expression of two key inflammatory mediators: tumor necrosis factor (Tnfa) and monocyte chemotactic protein 1 (Mcp1; also known as Ccl2). However, the chromatin
Melatonin induces histone hyperacetylation in the rat brain.
Niles, Lennard P, et al.
Neuroscience Letters (2013)
Judith K Davie et al.
Molecular and cellular biology, 22(3), 693-703 (2002-01-11)
The Tup1-Ssn6 complex regulates diverse classes of genes in Saccharomyces cerevisiae and serves as a model for corepressor functions in many organisms. Tup1-Ssn6 does not directly bind DNA but is brought to target genes through interactions with sequence-specific DNA binding
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4
Edmondson, D G, et al
Genes & Development, 10, 1247-1259 (1996)
Ramón Díaz-Trelles et al.
Nature communications, 7, 12088-12088 (2016-07-01)
Increasing angiogenesis has long been considered a therapeutic target for improving heart function after injury such as acute myocardial infarction. However, gene, protein and cell therapies to increase microvascularization have not been successful, most likely because the studies failed to
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