07-729

Sigma-Aldrich

Anti-CTCF Antibody

serum, Upstate®

Synonym(s):
11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, transcriptional repressor CTCF
eCl@ss:
32160702
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, primate, rat, canine

packaging

antibody small pack of 25 μL

mfr. no.

Upstate®

application(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

Gene Information

human ... CTCF(10664)

Related Categories

General description

CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.

Specificity

Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
Recognizes CTCF at a.a. 659-675.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.

Application

Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
Chromatin Immunoprecipitation:
An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.

Quality

Routinely evaluated by Western Blot.

Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).

Target description

Approx. 120 kDa

Linkage

Replaces: MABE547

Physical form

Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
HeLa nuclear extract.

Included Positive Antigen Control:
Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.

Other Notes

Concentration: The concentration of this serum antibody has not been determined.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Occupancy of chromatin organizers in the Epstein-Barr virus genome.
Holdorf, MM; Cooper, SB; Yamamoto, KR; Miranda, JJ
Virology null
M D Delgado et al.
FEBS letters, 444(1), 5-10 (1999-02-26)
CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human...
Friedreich ataxia patient tissues exhibit increased 5-hydroxymethylcytosine modification and decreased CTCF binding at the FXN locus.
Al-Mahdawi, S; Sandi, C; Mouro Pinto, R; Pook, MA
Testing null
Role of CCCTC binding factor (CTCF) and cohesin in the generation of single-cell diversity of protocadherin-? gene expression.
Monahan, K; Rudnick, ND; Kehayova, PD; Pauli, F; Newberry, KM; Myers, RM; Maniatis, T
Proceedings of the National Academy of Sciences of the USA null
Zhengang Peng et al.
PloS one, 7(5), e36891-e36891 (2012-05-23)
The cis-acting promoter element responsible for epigenetic silencing of retinoic acid receptor responder 1 (RARRES1) by methylation is unclear. Likewise, how aberrant methylation interplays effectors and thus affects breast neoplastic features remains largely unknown. We first compared methylation occurring at...

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