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07-748

Sigma-Aldrich

Anti-monomethyl-Histone H4 (Lys20) Antibody

Upstate®, from rabbit

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Synonym(s):
H4K20me1, Histone H4 (mono methyl K20)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

monomethylation (Lys20)

Gene Information

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This Item
05-67204-73507-1570
antibody form

purified antibody

antibody form

-

antibody form

culture supernatant

antibody form

affinity isolated antibody

clone

polyclonal

clone

6G7/H4, monoclonal

clone

NL314, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human, bovine, mouse, rat

species reactivity

human

species reactivity

human

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, multiplexing: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

multiplexing: suitable, western blot: suitable

technique(s)

ChIP: suitable, dot blot: suitable, inhibition assay: suitable (peptide), western blot: suitable

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Specificity

Broad species cross-reactivity expected due to sequence conservation
monomethylated Histone H4 (Lys20)

Immunogen

KLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[me1K]VLRDN) of human Histone H4

Application

Additional Referenced Research Applications:
Immunocytochemistry (cells)
Anti-monomethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20) & has been validated in WB, Mplex, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by immunoblot

Target description

10kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol.
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Li Liu et al.
PloS one, 8(11), e78253-e78253 (2013-11-14)
The upstream Gγ-globin cAMP-response element (G-CRE) plays an important role in regulating Gγ-globin expression through binding of ATF2 and its DNA-binding partners defined in this study. ATF2 knockdown resulted in a significant reduction of γ-globin expression accompanied by decreased ATF2

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