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07-760-I

Sigma-Aldrich

Anti-phospho-eIF-2α (Ser51)

Upstate®, from rabbit

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Synonym(s):
Eukaryotic translation initiation factor 2 subunit, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Drosophila (based on 100% sequence homology)

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

phosphorylation (pSer51)

Gene Information

Drosophila melanogaster ... Eif2Alpha(32617)

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E0157SAB4500729SAB5600109
vibrant-m

07-760-I

Anti-phospho-eIF-2α (Ser51)

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

NCBI accession no.

NP_001285329

NCBI accession no.

-

NCBI accession no.

P05198

NCBI accession no.

1965

technique(s)

ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:250 using human cerebral cortex sections , western blot: 1:250-1:500 using PC-12 cell lysate

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: 1:100-1:200, western blot: 1:100-1:200

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General description

Nuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5′-AACGACCGACCTTGAG-3′ of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).

Specificity

This rabbit polyclonal antibody detects elf-2 alpha phosphorylated on Serine 51 in human.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphorylated ser51 of human Eukaryotic translation initiation factor 2 subunit 1.

Application

Anti-phospho-eIF-2 (Ser51), Cat. No. 07-760-I, is a rabbit polyclonal antibody that detects Eukaryotic translation initiation factor 2 subunit 1 and has been tested for use in Peptide Inhibition Assay and Western Blotting.
Peptide Inhibition Assay: A 1:1,000 dilution from a representative lot detected phospho-eIF-2 (Ser51) in HeLa treated Calyculin A.
Research Category
Signaling

Quality

Evaluated by Western Blotting in HeLa treated with Calyculin A cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eIF-2 (Ser51) in HeLa treated with Calyculin A cell lysate.

Target description

~38 kDa observed; 38.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kristin L Rosche et al.
mSphere, 8(5), e0032123-e0032123 (2023-09-21)
A crucial phase in the life cycle of tick-borne pathogens is the time spent colonizing and persisting within the arthropod. Tick immunity is emerging as a key force shaping how transmissible pathogens interact with the vector. How pathogens remain in

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