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07-909

Sigma-Aldrich

Anti-phospho-Src (Tyr418) Antibody

Upstate®, from rabbit

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Synonym(s):
c-Src, p60-Src Src kinase
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

chicken, human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr418)

Gene Information

human ... SRC(6714)

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This Item
04-85705-67705-184-AF488
antibody form

affinity purified immunoglobulin

antibody form

culture supernatant

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

2N8, monoclonal

clone

9A6, monoclonal

clone

GD11, monoclonal

species reactivity

chicken, human, mouse

species reactivity

vertebrates, mouse

species reactivity

mouse, human

species reactivity

rat, human, mink, mouse, avian

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable

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Specificity

Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.
Recognizes Src phosphorylated on Tyrosine residue 418.

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)

Application

Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunoblot.

Target description

60 kDa

Physical form

Affinity Purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Aida Habibovic et al.
JCI insight, 1(18), e88811-e88811 (2016-11-05)
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Functional stability of a mixed microbial consortium producing PHA from waste carbon sources.
Erik R Coats,Frank J Loge,William A Smith,David N Thompson,Michael P Wolcott
Applied Biochemistry and Biotechnology null
Src: regulation, role in human carcinogenesis and pharmacological inhibitors.
Tsygankov, A Y and Shore, S K
Current Pharmaceutical Design, 10, 1745-1756 (2004)
c-Src and cooperating partners in human cancer
Ishizawar, Rumey and Parsons, Sarah J
Cancer Cell, 6, 209-214 (2004)
Shinkyu Choi et al.
Antioxidants & redox signaling, 30(4), 505-519 (2018-01-18)
Altered redox state has been related to the development of normal pregnancy (NP) and preeclampsia (PE). Endothelial KCa2.3 and KCa3.1 (KCas) play an important role in vasodilation, and KCas levels are affected by oxidative stress. We investigated the mechanisms of

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