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Sigma-Aldrich

Anti-phospho-mTOR (Ser2448) Antibody

Upstate®, from rabbit

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Synonym(s):
FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

phosphorylation (pSer2448)

Gene Information

human ... MTOR(2475)

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Anti-mTOR Antibody, clone 21D8.2 clone 2ID8.2, from mouse

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Anti-mTOR Antibody, clone 21A12.2 clone 21A12.2, from mouse

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Anti-mTOR Antibody, clone 21A12.2

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

polyclonal

clone

polyclonal

clone

2ID8.2, monoclonal

clone

21A12.2, monoclonal

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

mouse, human

species reactivity

human

Gene Information

human ... MTOR(2475)

Gene Information

human ... MTOR(2475)

Gene Information

human ... MTOR(2475)

Gene Information

human ... MTOR(2475)

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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General description

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

Specificity

Predicted to cross react with rat, mouse, sheep & Rhesus Macaque based on 100% sequence homology.
Recognizes mTOR when phosphorylated on Ser2448

Immunogen

Amino acids encompassing and including phosphorylated Ser2448 of human mTOR

Application

Research Sub Category
PI3K, Akt, & mTOR Signaling

Glucose/Glycogen Metabolism

Insulin/Energy Signaling

Adhesion (CAMs)
This Anti-mTOR Antibody is validated for use in WB for the detection of phospho-mTOR (Ser2448).

Quality

Evaluated by western blot on EGF-treated and untreated A431 cell lysate.

Western Blot Analysis:
This antibody detected mTOR phosphorylated on Ser2448 in EGF-treated and untreated A431 cell lysates.

Target description

~289 kDa observed.
An uncharacterized band may be obsereved at ~59 kDa

Physical form

Purified rabbit polyclonal IgG in storage buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
EGF-treated and untreated A431 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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