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09-217

Sigma-Aldrich

Anti-Raptor Antibody

Upstate®, from rabbit

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Synonym(s):
p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... RPTOR(57521)

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This Item
05-147005-1564ABS88
Anti-Raptor Antibody Upstate®, from rabbit

Sigma-Aldrich

09-217

Anti-Raptor Antibody

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

serum

clone

polyclonal

clone

1H6.2, monoclonal

clone

21A12.2, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

human

species reactivity

human

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

Specificity

Other species have not been tested.

Immunogen

KLH conjugated peptide encompassing the N-terminal region of human Raptor.

Application

Detect Raptor using this anti-Raptor Antibody validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Routinely evaluated by immunoblot.

Target description

150 kDa

Physical form

Affinity purified rabbit polyclonal IgG in 0.02M PBS, 0.25M sodium chloride containing 0.1% sodium azide and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Dos D Sarbassov et al.
The Journal of biological chemistry, 280(47), 39505-39509 (2005-09-27)
The raptor-mTOR protein complex is a key component of a nutrient-sensitive signaling pathway that regulates cell size by controlling the accumulation of cellular mass. How nutrients regulate signaling through the raptor-mTOR complex is not well known. Here we show that
Hiroki Nojima et al.
The Journal of biological chemistry, 278(18), 15461-15464 (2003-02-27)
The mammalian target of rapamycin (mTOR) controls multiple cellular functions in response to amino acids and growth factors, in part by regulating the phosphorylation of p70 S6 kinase (p70S6k) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1). Raptor (regulatory associated
Gregory A Wyant et al.
Cell, 171(3), 642-654 (2017-10-21)
The mTORC1 kinase is a master growth regulator that senses many environmental cues, including amino acids. Activation of mTORC1 by arginine requires SLC38A9, a poorly understood lysosomal membrane protein with homology to amino acid transporters. Here, we validate that SLC38A9
Do-Hyung Kim et al.
Cell, 110(2), 163-175 (2002-08-02)
mTOR/RAFT1/FRAP is the target of the immunosuppressive drug rapamycin and the central component of a nutrient- and hormone-sensitive signaling pathway that regulates cell growth. We report that mTOR forms a stoichiometric complex with raptor, an evolutionarily conserved protein with at
Kenta Hara et al.
Cell, 110(2), 177-189 (2002-08-02)
mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of raptor

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