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09-230

Sigma-Aldrich

Anti-phospho-Raptor (Thr706) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
P150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

canine, human

species reactivity (predicted by homology)

horse (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), dog (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

dot blot: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr706)

Gene Information

bovine ... Rptor(507056)
human ... RPTOR(57521)

General description

Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.

Specificity

This antibody recognizes Raptor phosphorylated at Thr706.

Immunogen

Epitope: Ser706
KLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Thr706.

application

Detect phospho-Raptor (Thr706) using this Anti-phospho-Raptor (Thr706) Antibody validated for use in WB, DB.

Quality

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein.

Dot Blot Analysis: 1 µg/mL of this antibody detected Raptor in modified and non-modified peptides of phosphorylated and non-phosphorlyated Raptor protein.

Target description

~ 150 kDa

Physical form

Purified rabbit serum in PBS with 0.05% sodium azide.

Analysis Note

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Shijie Ma et al.
Oncogene, 41(17), 2431-2443 (2022-03-14)
eIF3a (eukaryotic translation initiation factor 3a), a subunit of the eIF3 complex, has been suggested to play a regulatory role in protein synthesis and in cellular response to DNA-damaging treatments. S6K1 is an effector and a mediator of mTOR complex

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