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Sigma-Aldrich

Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25)

serum, from rabbit

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Synonym(s):
dimethyl-arginine, asymmetric
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

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07-41307-412ABS535
vibrant-m

ABS535

Anti-Arginase-1 Antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse

species reactivity

human, mouse

species reactivity

mouse, human

species reactivity

guinea pig, human, mouse, rat

antibody form

serum

antibody form

purified antibody

antibody form

serum

antibody form

purified immunoglobulin

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

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General description

Asymmetrical dimethlarginine (ADMA) is an endogenous, L-arginine metabolite arising from the proteolysis of methylated arginine containing proteins and is a known inhibitor of nitric oxide synthase (NOS). In various studies, ADMA has been observed as having a regulatory role in cell motility and the actin cytoskeleton. Elevated levels of ADMA are associated with sporadic FSGS, coronary artery disease, hypertension, peripheral arterial occlusive disease, obesity, stroke, preeclampsia, and cardiovascular mortality/morbidity in patients with diabetic nephropathy.

Specificity

This antibody recognizes dimethylated arginines.

Immunogen

KLH-conjugated linear peptide containing asymmetric dimethyl-arginine-glycine repeats.

Application

Detect dimethyl-Arginine using this Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25) validated for use in WB.

Quality

Evaluated by Western Blot in PRMT1 treated and untreated MEF cell lysates.

Western Blot Analysis: 1:1,000 dilution of this antibody detected dimethyl-Arginine on 10 µg of PRMT1 treated and untreated MEF cell lysates.

Target description

Multiple. This antibody detects proteins containing asymmetrical dimethylated arginines.

Analysis Note

Control
PRMT1 treated and untreated MEF cell lysates

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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