100065

Sigma-Aldrich

2-APB - CAS 524-95-8 - Calbiochem

A cell-permeable modulator of Ins(1,4,5)P3-induced Ca2+ release.

Synonym(s):
2-APB - CAS 524-95-8 - Calbiochem, 2-Aminoethoxydiphenylborate, (2-Aminoethoxy)diphenylborane
Empirical Formula (Hill Notation):
C14H16BNO
CAS Number:
Molecular Weight:
225.09
MDL number:
NACRES:
NA.77

storage conditions

+2C to +8C

Quality Level

assay

≥97% (titration)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

white

solubility

DMSO: 20 mg/mL
95% ethanol: 25 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C14H16BNO/c16-11-12-17-15(13-7-3-1-4-8-13)14-9-5-2-6-10-14/h1-10H,11-12,16H2

InChI key

BLZVCIGGICSWIG-UHFFFAOYSA-N

General description

A cell-permeable modulator of Ins(1,4,5)P3-induced Ca2+ release. Inhibits Ins(1,4,5)P3-induced Ca2+ release from rat cerebellar microsomal preparations (IC50 = 42 µM) without affecting [3H]-Ins(1,4,5)P3 binding to its receptor. In liver cells, 2-APB inhibits store-operated Ca2+ channels through a mechanism which may involve its binding to either the channel protein or an associated regulatory protein. Has no effect on the Ca2+ release from the ryanodine-sensitive Ca2+ store prepared from rat leg skeletal muscle and heart.
A cell-permeable modulator of Ins(1,4,5)P3-induced Ca2+ release. Inhibits Ins(1,4,5)P3-induced Ca2+ release from rat cerebellar microsomal preparations (IC50 = 42 µM) without affecting [3H]-Ins(1,4,5)P3 binding to its receptor. Does not affect Ca2+ release from the ryanodine-sensitive Ca2+ store in rat leg skeletal muscle and heart. Inhibits spontaneous as well as the phasic contractions induced by uterotonic agents. Also reported to inhibit angiotensin II-induced contractions in aortic muscles.

Packaging

100 mg in Glass bottle
Packaged under inert gas

Biochem/physiol Actions

Cell permeable: yes
Target IC50: 42 µM against Ins(1,4,5)P3-induced Ca2+ release from rat cerebellar microsomal preparations
Product does not compete with ATP.
Reversible: no
Primary Target
Ins(1,4,5)P3-induced Ca2+ release

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Brooke, R. T., et al. 2004. Lab. Invest.84, 29.
Gregory, R.B., et al. 2001. Biochem. J.354, 285.
Ma, H.T., et al. 2001. J. Biol. Chem.276, 18888.
Ascher-Landsberg, S., et al. 1999. Biochem. Biophys. Res. Commun.264, 979.
Maruyama, T., et al. 1997. J. Biochem.122, 498.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Target Organs

Respiratory system

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Goli Ardestani et al.
Cell calcium, 87, 102181-102181 (2020-02-26)
Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca2+) influx and spontaneous Ca2+ oscillations. The oscillations cease during maturation but Ca2+ influx continues, as the oocytes' internal stores attain...
Hui Zhang et al.
Cell host & microbe, 27(4), 556-570 (2020-03-07)
The discovery of TMEM173/STING-dependent innate immunity has recently provided guidance for the prevention and management of inflammatory disorders. Here, we show that myeloid TMEM173 occupies an essential role in regulating coagulation in bacterial infections through a mechanism independent of type...
Miriam Hernández-Morales et al.
Cell reports, 30(10), 3250-3260 (2020-03-12)
One approach to magnetogenetics uses radiofrequency (RF) waves to activate transient receptor potential channels (TRPV1 and TRPV4) that are coupled to cellular ferritins. The mechanisms underlying this effect are unclear and controversial. Theoretical calculations suggest that the heat produced by...

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