The bottle contains sufficient powder to prepare 250 mL of medium. Please see the link below to the product information sheet. For preparation instructions, refer to page two.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/119/781/100188dat-ms.pdf
1.00188
Chromocult® RAMBACH™ Agar
ISO 6579, Specialized media for the isolation and differentiation of Salmonella spp. from food and animal feed, water and other materials.
Synonym(s):
Chromogenic Salmonella Agar, Propylene Glycol Deoxycholate Neutral Red Agar, Salmonella Agar
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4 X 250 ML
$132.00
4 X 1000 ML
$449.00
About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
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agency
ISO 6579
suitable for ISO
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.3±0.2 (25 °C)
application(s)
food and beverages
storage temp.
15-25°C
suitability
selective for enterobacteriaceae: Salmonella spp.
Related Categories
1 of 4
This Item | 1.46719 | 1.00850 | 1.10747 |
|---|---|---|---|
| form powder | form (Solid in plate) | form granular | form granular |
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability selective for enterobacteriaceae: Salmonella spp. | suitability Salmonella spp. | suitability Escherichia coli, coliforms | suitability Salmonella spp. (spp.) |
| agency ISO 6579, suitable for ISO | agency ISO 6579 | agency - | agency ISO 6579 |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages, microbiology | application(s) food and beverages |
| pH 7.3±0.2 (25 °C) | pH 7.3 ( in H2O) | pH 6.8-7.2 (25 °C, 34.6 g/L in H2O) | pH 6.7-7.1 (37 °C, 52 g/L in H2O, after autoclaving) |
General description
Chromocult® RAMBACH™ Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Application
The Chromocult® RAMBACH™ Agar can be used for: -
- The detection of Salmonella in foodstuff, water, animal feed
- The differential diagnosis of Salmonella species in various samples
Analysis Note
- The dehydrated medium appears as dark-green color.
- The prepared medium is opaque and light pink in appearance.
- The pH value at 25 degrees is in the range of 7.1-7.5.
Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Rambach is a trademark of Balel S.A.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
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Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
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Is it possible to give us some information about Chromcult Rambach Agar: the pack size is 4 x 250ml but the form is powder? How do we prepare this agar?
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