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NutriSelect® prime Bismuth Sulfite (BS) Agar

acc. to Wilson and Blair

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Synonym(s):
BSA, WILSON BLAIR Agar, Wismuth sulfite agar

Agency

ISO 6579-1
USP 31

Quality Level

description

FDA-BAM

form

powder

technique(s)

microbiological culture: suitable

pH

7.7±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

2-8°C

suitability

selective and differential for enterobacteriaceae: Salmonella spp.

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This Item
1.03853736081.05418
BiGGY Agar suitable for microbiology, NutriSelect® Plus

Millipore

73608

BiGGY Agar

form

powder

form

powder

form

powder

form

powder (dehydrated (DCM))

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

7.7±0.2 (25 °C)

pH

7.0±0.2 (25 °C)

pH

-

pH

7.6 (37 °C, 47 g/L in H2O, after autoclaving)

application(s)

food and beverages
pharmaceutical

application(s)

food and beverages
pharmaceutical

application(s)

agriculture
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
veterinary, microbiology

application(s)

clinical
microbiology

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

General description

NutriSelect® Bismuth Sulfite (BS) Agar introduced by Wilson-Blair (1927) is specifically designed for the isolation and differentiation of Salmonella spp from food, animal feed and other materials precisely when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of concern.
Colonies of H2S positive Salmonella exhibit blackening due to the formation of iron sulfide. The media selectivity is enhanced by the presence of brilliant green and bismuth sulfite which inhibit the growth of gram-positive bacteria and coliforms in the sample.
Peptone, meat extract and glucose allow good growth. Disodium phosphate acts as a buffer to maintain pH and agar is the solidifying agent.

Application

NutriSelect® Bismuth Sulfite (BS) Agar is used to isolate and differentiate Salmonella spp. from food animal feed and other materials. The medium is used to selectively grow the species when S. typhi and S. paratyphi are expected.

Analysis Note

Salmonella colonies display blackening after 18 hrs of incubation.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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