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1.00212

Millipore

Selenite Cystine Broth

for enrichment of Salmonella

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Synonym(s):
Selenite-cystine broth

Agency

ISO

Quality Level

reg. compliance

FDA

form

medium granules

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

6.8-7.2 (37 °C, 23 g/L in H2O)

solubility

23 g/L

bulk density

900 kg/m3

application(s)

clinical
food and beverages
industrial qc
microbiology

storage temp.

2-8°C

suitability

Salmonella spp.

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1.096281.054431.10824
Selenite Cystine Broth for enrichment of Salmonella

Millipore

1.00212

Selenite Cystine Broth

form

medium granules

form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

6.8-7.2 (37 °C, 23 g/L in H2O)

pH

7.1-7.5 (25 °C in H2O)

pH

7.0 (37 °C, 8 g/L in H2O, after autoclaving)

pH

7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving)

solubility

23 g/L

solubility

-

solubility

8 g/L

solubility

54.4 g/L

General description

GranuCult® prime Selenite Cystine Broth is for the selective enrichment of Salmonella from food and animal feed and other materials, especially when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of specific concern.
Peptones including the enzymatic digest of casein provide nitrogen, vitamins, amino acids and Lactose provides the carbon sources. Disodium phosphate acts as a buffer to maintain the pH.
Selenite inhibits the growth of coliform bacteria and enterococci in the first 6-12 hours of incubation, its inhibitory effect then gradually declines.
L-Cystine is considered to enhance the growth of Salmonella by reduction of toxicity. According to the modification described by North and Bartram (1953), L-Cystine is added to the original formulation of Leifson (1936).

Application

GranuCult® prime Selenite Cystine Broth is used for the selective enrichment of Salmonella from food and animal feed and other materials, especially when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of specific concern.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Supplementary Hazards

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3


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