1.00212

Selenite Cystine Broth

GranuCult^® prime, according to ISO 19250, FDA-BAM, AOAC^®, APHA, granular, selective for Salmonella spp., pack of 500 g

• Synonym(s): Salmonella selective broth, Selenite Cystine Medium, Selenite-cystine broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: AOAC^®; APHA; FDA-BAM; according to ISO 19250
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pack of 500 g
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 6.8-7.2 (37 °C, 23 g/L in H₂O)
• solubility: 23 g/L
• bulk density: 900 kg/m³
• application(s): clinical; food and beverages; industrial qc; microbiology
• storage temp.: 2-8°C
• suitability: selective for Salmonella spp.

Description

General description

GranuCult^® prime Selenite Cystine Broth is for the selective enrichment of Salmonella from food and animal feed and other materials, especially when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of specific concern.
Peptones including the enzymatic digest of casein provide nitrogen, vitamins, amino acids and Lactose provides the carbon sources. Disodium phosphate acts as a buffer to maintain the pH.
Selenite inhibits the growth of coliform bacteria and enterococci in the first 6-12 hours of incubation, its inhibitory effect then gradually declines.
L-Cystine is considered to enhance the growth of Salmonella by reduction of toxicity. According to the modification described by North and Bartram (1953), L-Cystine is added to the original formulation of Leifson (1936).

Application

GranuCult^® prime Selenite Cystine Broth is used for the selective enrichment of Salmonella from food and animal feed and other materials, especially when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of specific concern.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

AOAC is a registered trademark of AOAC International

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS06
• signalword: Danger
• hcodes: H300 + H330 - H315 - H317 - H319 - H412
• pcodes: P260 - P273 - P280 - P302 + P352 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• supp_hazards: EUH031
• Storage Class: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3