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1.00497
AFB-Color modified
Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method
Recommended Products
Quality Level
IVD
for in vitro diagnostic use
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
suitability
(Acid-fast bacteria)
Related Categories
1 of 4
This Item | 1.01603 | 1.32657 | 1.09215 |
|---|---|---|---|
| application(s) clinical testing | application(s) clinical testing | application(s) clinical testing | application(s) clinical testing |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| suitability (Acid-fast bacteria) | suitability - | suitability - | suitability - |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
General description
AFB-Color modified staining set uses the classical Ziehl-Neelsen hot staining procedure and can be used for staining on the staining rack. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. This AFB-Color modified staining kit uses methylene blue as counterstain.
Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material.
The package is sufficient for approx. 250 applications. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
Analysis Note
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
69.8 °F
Flash Point(C)
21 °C
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