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1.00567

Sigma-Aldrich

Lugol′s solution

stabilized with PVP for the Gram staining

Quality Level

form

liquid

IVD

for in vitro diagnostic use

application(s)

hematology: suitable
histology: suitable

pH

2.0 (20-22 °C in H2O)

density

1.02 g/cm3 at 20 °C

Featured Industry

Clinical Testing
Diagnostic Assay Manufacturing

storage temp.

15-25°C

General description

The Lugol′s solution stabilized with PVP, is a ready-to-use solution used in bacteriology, for example, for the staining of Gram bacteria. It contains a complex of Iodine-Polyvinylpyrrolidon (PVP) and no free iodine, which stabilizes the solution and reduces the evaporation of iodine. During Gram-staining, the iodine from the Lugol′s solution and crystal violet (Cat. no. 109218) form a stable complex that will not be released by decolorisation. Thus, Gram-positive bacteria will appear blue-violet when analyzed microscopically, wheras Gram-negative bacteria can be counterstained with a safranine solution (Product number 1.09217) and will appear pink to red.
1 L of the Lugol′s solution stabilized with PVP is capable of staining up to 500 slides. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
gram-positve microorganisms: blue-violet
gram-negative microorganisms: pink to red

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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