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1.01301

Sigma-Aldrich

Auramine O (C.I. 41000)

for microscopy

Synonym(s):
Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
Empirical Formula (Hill Notation):
C17H22ClN3
CAS Number:
Molecular Weight:
303.83
MDL number:
EC Index Number:
219-567-2

Quality Level

form

solid

potency

1000 mg/kg LD50, oral (Rat)

pH

6-7 (20 °C, 10 g/L in H2O)

mp

265 °C (decomposition)

solubility

10 g/L

bulk density

430 kg/m3

εmax

1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

General description

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

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