1.01368

Brilliant cresyl blue zinc chloride double salt

for microscopy Certistain^®

• Synonym(s): Brilliant cresyl blue zinc chloride double salt
• Linear Formula: (C₁₇H₂₀N₃O)₂ · ZnCl₄
• CAS Number: 51716-96-2
• Molecular Weight: 771.92
• MDL number: MFCD00148901
• EC Index Number: 257-358-8

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥60% spectrophotometric assay
• pH: 5.5 (20 °C, 5 g/L in H₂O)
• mp: 195-198 °C
• solubility: 30 g/L
• bulk density: 560 kg/m³
• εmax: ≥1500 at 623-628 nm in 50% ethanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: SIHASHFMDFXGSX-UHFFFAOYSA-J

Description

General description

Brilliant cresyl blue zinc chloride double salt for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution, that makes target structures (by staining with the above brilliant cresyl blue solution) evaluable for diagnostic purposes.The regenerative capacity of erythrocytes can be detected by counting of reticulocytes. The measurement of substantia granulofilamentosa (ribonucleoproteins) is possible with fresh, non-fixed, young erythrocytes (supravital staining).Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly. When stained with brilliant cresyl blue the reticulocytes display a black-blue network or black-blue dots.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Packaging

25 , 25 g

1 kg

Analysis Note

Dye content (spectrophotometrically): ≥ 60% C₁₇H₂₀N₃O
Identity
- UV/VIS-Spectrum:
passes test
- Zinc: passes test
Absorption maximum λ max (ethanol 50 %): 623 - 628 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; ethanol 50 %): ≥ 1500
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(C): Not applicable