1.01368

Sigma-Aldrich

Brilliant cresyl blue zinc chloride double salt

for microscopy Certistain®

Synonym(s):
Brilliant cresyl blue zinc chloride double salt
Linear Formula:
(C17H20N3O)2 · ZnCl4
CAS Number:
Molecular Weight:
771.92
MDL number:
EC Index Number:
257-358-8

storage conditions

Store at +5°C to +30°C.

Quality Level

form

solid

composition

Dye, ≥60% spectrophotometric assay

application(s)

hematology: suitable
histology: suitable

pH

5.5 (20 °C, 5 g/L in H2O)

mp

195-198 °C

solubility

30 g/L

bulk density

560 kg/m3

εmax

≥1500 at 623-628 nm in 50% ethanol at 0.005 g/L

Featured Industry

Diagnostic Assay Manufacturing

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

General description

Brilliant Cresyl Blue Zinc Chloride Double Salt is a dry dye of the series of Certistain dyes that is used for the preparation of a staining solution that can stain reticulocytes in blood. It is a CERTISTAIN dye that is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Analysis Note

Dye content (spectrophotometrically): ≥ 60% C₁₇H₂₀N₃O
Identity
- UV/VIS-Spectrum:
passes test
- Zinc: passes test
Absorption maximum λ max (ethanol 50 %): 623 - 628 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %): ≥ 1500
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

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