This product is prepared as a concentrate. Please see the link below to the Data Sheet for the dilution instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/412/029/101424ug-ms.pdf
1.01424
May-Grünwald′s eosine-methylene blue solution modified
for microscopy, suitable for microscopy
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100 ML
$37.50
500 ML
$65.00
1 L
$113.00
2.5 L
$196.00
25 L
$1,660.00
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100 ML
$37.50
500 ML
$65.00
1 L
$113.00
2.5 L
$196.00
25 L
$1,660.00
About This Item
UNSPSC Code:
41116130
EC Index Number:
200-659-6
NACRES:
NA.05
vapor pressure
128 hPa ( 20 °C)
Quality Level
form
liquid
autoignition temp.
420 °C
composition
C.I. 45380, 0.5 g/L
C.I. 52015, 0.7 g/L
contains CH3OH
expl. lim.
5.5-44 % (v/v) Methanol)
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
bp
65 °C/1013 hPa
transition temp
flash point 12 °C
density
0.79 g/cm3 at 20 °C
suitability
suitable for microscopy
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
1 of 4
This Item | 1.01352 | 1.09204 | 1.01383 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - |
| grade for microscopy | grade - | grade for microscopy | grade - |
| application(s) clinical testing | application(s) diagnostic assay manufacturing | application(s) clinical testing | application(s) clinical testing |
| form liquid | form solid | form liquid | form liquid |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| storage temp. 15-25°C | storage temp. 2-30°C | storage temp. 15-25°C | storage temp. 15-25°C |
General description
The staining method according to May-Grünwald is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue solution and Leishman′s eosin methylene blue solution, this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The 500 ml bottle provides ca. 1500 stainings. The May-Grünwald′s eosin methylene blue solution is an IVD / CE registered / certified product and the 500 ml bottle is sufficient for staining up to 1600 slides in Hellendahl cells. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Suitability for microscopy (Blood smear): passes test
Erythrocytes: pink to brownish
Nuclei: violet
Eosinophilic granules: red-brown
Neutrophilic granules: light violet
Lymphocyte cytoplasm: blue
Erythrocytes: pink to brownish
Nuclei: violet
Eosinophilic granules: red-brown
Neutrophilic granules: light violet
Lymphocyte cytoplasm: blue
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk_germany
WGK 2
flash_point_f
53.6 °F
flash_point_c
12 °C
Certificates of Analysis (COA)
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should this be used neat or diluted with methanol and/or buffered water
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