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1.01597

Sigma-Aldrich

AFB-Fluor phenol-free

Staining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)

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Quality Level

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

detection method

fluorometric (microscopy)

storage temp.

15-25°C

General description

The decisive feature of the AFB-Fluor phenol-free for detection of aldehyde and mucosubstances, is the presence of fluorescence dyes in the staining solution. This enables the swift and secure identification of the acid-fast bacteria under the fluorescence microscope. As it has become an established procedure alongside Ziehl-Neelson staining (AFB-Color modified), we provide three ready-to-use solutions in our AFB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Product number 1.00497) (ZN) is possible. The AFB-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.
The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Mycobacteria: red-orange/yellow-green
Background: dark

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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