MilliporeSigma
All Photos(1)

1.01647

Sigma-Aldrich

Alcian blue solution

for microscopy

Sign Into View Organizational & Contract Pricing

Select a Size

Quality Level

form

liquid

IVD

for in vitro diagnostic use

pH

2.5 (25 °C in H2O)

density

1.006 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
1.326571.326471.00425
Alcian blue solution for microscopy

Sigma-Aldrich

1.01647

Alcian blue solution

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

pH

2.5 (25 °C in H2O)

pH

2.5

pH

1.0

pH

2.5

density

1.006 g/cm3 at 20 °C

density

-

density

-

density

-

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

The Alcian blue solution, pH 2.5 - for microscopy, is a ready-to-use staining solution used for human-medical cell diagnosis and serves the histological investigation of sample material of human origin, for example histological sections of e. g. the intestine or stomach. The present ready-to-use Alcian blue solution, pH 2.5 can be used for the staining of acid mucopolysaccharides in histological tissue. Acidic mucosubstances are selectively stained in the tissue, after which the specimen is counterstained with nuclear fast red. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution (Cat. no.100121). The alcian blue staining method is frequently combined with the Periodic acid-Schiff (PAS) reaction. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins and glycol- and phospholipids can be visualized in addition.
The 500 ml bottle is sufficient for 2000 - 3000 stainings.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
neutral mucopolysaccharides: purple
polysaccharides: purple
PAS negative acidic mucosubstances: light blue
Nuclei: blue

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service