1.01800

Alkaline Peptone Water

According to ISO 8914, FDA-BAM, APHA, and AOAC; For microbiology

• Synonym(s): APW; Peptone Water, alkaline; Peptone Water
• NACRES: NA.74
• UNSPSC Code: 41106213
• Manufacturer/tradename: GranuCult^® prime
• Agency: AOAC^®, APHA, FDA-BAM, ISO 8914
• Ph: 8.3-8.7 (25 °C, 20 g/L in H₂O, after autoclaving)
• Form: granular
• Suitability: Vibrio spp.
• Application(s): food and beverages; microbiology

Properties

• Quality Level: 200
• agency: AOAC^®, APHA, FDA-BAM, ISO 8914
• sterility: non-sterile
• form: granular
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 8.3-8.7 (25 °C, 20 g/L in H₂O, after autoclaving)
• solubility: 20 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Vibrio spp.

Description

General description

Alkaline Peptone Water is recommended for the enrichment and isolation of Vibrio cholerae and Vibrio species. The growth of a broad spectrum of Vibrio sp. is promoted by peptones, a sodium chloride concentration of 10 g/liter, and a high pH of 8.5. The alkaline pH of this medium allows the growth of Vibrio species, while it inhibits the growth of other unwanted flora.

Application

Alkaline Peptone Water is used for the enrichment of Vibrio cholerae and Vibrio sp. in food and other materials.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellow
pH-value (25 °C): 8.3 - 8.7
Colony count (Vibrio vulnificus ATCC 33149): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Inaba CH 38): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Ogawa CH 60): ≥ 1E+06 CFU/ml
Colony count (Vibrio parahaemolyticus ATCC 17802 (WDCM 00037)): ≥ 1E+06 CFU/ml
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: bloog agar.

Legal Information

AOAC is a registered trademark of AOAC International

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3