1.03795

Cellvento^® 4CHO COMP

chemically defined cell culture medium, ≤1.0 IU/mL endotoxin, pH 5.1-5.6(without supplement), pH 6.7-7.3(with supplement)

• UNSPSC Code: 41106213

Properties

• Quality Level: 500
• description: shipped with cooling packs
• product line: Cellvento^®
• form: dry powder
• technique(s): cell culture | mammalian: suitable (suspension)
• storage temp.: 2-8°C

Description

General description

Cellvento^® 4CHO chemically defined cell culture medium has been specially developed for the growth of Chinese Hamster Ovary (CHO) cells and the expression of monoclonal antibodies and recombinant proteins in suspension culture. The formulation is of non-animal origin, chemically defined and contains no hydrolysates or components of unknown composition. Cellvento^® 4CHO medium has been formulated without L-glutamine.

Application

Cellvento^® 4CHO medium should be used as amplification medium and production medium in fed-batch applications. It is recommended to combine it with the companion feed supplement Cellvento^® 4Feed in fed-batch manufacturing processes. The medium does not contain hypoxanthine and thymidine to allow a broad utilization, also in dhfr- transfected cells. The feed does not contain glucose to allow a fine tuning of the glucose concentration during fed-batch processes, thus allowing to minimize lactate production. The feed contains sources of both cysteine and tyrosine and should not be supplemented with any additional alkaline feed. This product is intended for research or further manufacturing but not for human or therapeutic use

Quantity

Formulated to contain 23.7 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317 - H319
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2